XTIMATE® SERIES HPLC COLUMN
- Next generation beyond mid-range priced Ultisil® series
Xtimate® HPLC column derives its outstanding performance from a special hybrid particle based technique, which coats a unique 2nm organic/inorganic polymer layer on the silica surface, so that the pH range is extended to 1.0-12.5. Xtimate column is designed for HPLC method development. Regardless of the types of mobile phase or high temperature, Xtimate® HPLC column always has stable performance and long lifetime.
Features
- EXtra pH range: wide pH range from 1.0 to 12.5, excellent peak shape for strong bases.
- EXtra column lifetime: 5 times of similar product such as Gemini.
- EXtra performance: column efficiency of 5um columns is as high as 90000/m, 2-3 times of that of Xterra.
- EXtra care from Welch: enjoy excellent pre-sales and after-sales service from Welch.
| Column: | Xtimate® C18, 5um, 150×4.6mm |
| Mobile Phase: | CH3CN/0.01N-NaOHaq.(pH=12)=30/70 |
| Flow Rate: | 1.0ml/min |
| Temperature: | 30°C |
| Soak Time: | 4 hours |
| Column: | Ultisil® XB-C18, 5um, 150x4.6mm |
| Mobile Phase: | CH3OH/H2O=60/40 |
| Flow Rate: | 1.0ml/min |
| Temperature: | 40°C |
| Soak Time: | UV 254nm |
| Samples: | 1) Uracil 2) Methyl benzoate |
| 3) Toluene 4) Naphthalene |
Unprecedented Peak Shape
At mid pH, strong bases usually exhibit bad tailing due to secondary interactions between the analytes and the surface silaols. In Welch’s unique technique, the hybrid layer totally covers the surface silanols and blocks analytes access to these surface silanols. Improved bonding and endcapping further reduce silanol activity. As a result, hybrid particle based Xtimate® columns show unprecedented peak shape.
Notes:
Figure 1: The detection of amitriptyline by poor
endcapping product
Figure 2: The detection of amitriptyline by Xtimate® C18
Figure 3: The detection of Amitriptyline by Symmetry C18
XTIMATE® C18
| Structural Formula |  |
| pH Range | 1.0-12.5 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | 14(120Å), |
| USP List | L1 |
| Endcapped | Yes |
XTIMATE® C8
| Structural Formula |  |
| pH Range | 1.0-12.5 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å), 100(300Å) |
| Carbon Loading (%) | 10(120Å), 5(300Å) |
| USP List | L7 |
| Endcapped | Yes |
XTIMATE® C4
| Structural Formula |  |
| pH Range | 1.0-12.5 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å), 100(300Å) |
| Carbon Loading (%) | 14(120Å), 4(300Å) |
| USP List | L26 |
| Endcapped | Yes |
XTIMATE® Phenyl-Hexyl
| Structural Formula |  |
| pH Range | 1.0-12.5 |
| Particle Size | 3µm, 5µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | 12(120Å) |
| USP List | L11 |
| Endcapped | Yes |
XTIMATE® CN
| Structural Formula |  |
| pH Range | 1.0-12.5 |
| Particle Size | 5µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | 7(120Å), |
| USP List | L10 |
| Endcapped | Yes |
XTIMATE® Polar-RP
| Structural Formula |  |
| pH Range | 1.0-12.5 |
| Particle Size | 5µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | 16(120Å) |
| USP List | L1 |
| Endcapped | Yes |
XTIMATE® POLYMER SERIES HPLC COLUMN
XTIMATE® Sugar-H
| pH Range | 1.0-3.0 |
| Particle Size | 5µm, 8µm |
| Cross-link | 8% |
| Counter lon | H3. |
| USP List | L17 |
| Max. Temp. | 95C |
XTIMATE® Sugar-Ca
| pH Range | 5.0-9.0 |
| Particle Size | 5µm, 8µm |
| Cross-link | 8% |
| Counter lon | Ca2+ |
| USP List | L19 |
| Max. Temp. | 95C |
XTIMATE® PS/DVB
| pH Range | 1.0-14.0 |
| Particle Size | 5µm, 10µm |
| Surface Area(m2/g) | 450(300Ā) |
| USP List | L21 |
| Max. Temp. | 75C |
XTIMATE® SEC SERIES HPLC COLUMN
Xtimate® SEC
Xtimate® SEC (size exclusion chromatography), also known as “global protein hydrophilic modified silica column”, is made from ultra-high purity, stable silica bonded with hydrophilic polymer and diol functional groups. This double bonding mechanism, which makes possible of nonspecific adsorption of high Mw polymers, proteins, biological enzymes, polypep- tides and other biological samples, can be applied to separating water-soluble polymers from biomacromolecules.
Features
- Ultra-high purity, stable silica bonded with hydrophilic polymer and diol functional groups. shape for strong bases.
- 5um or 3um silica microsphere, high separation efficiency.
- 120Å minibore columns fit for analysis of polar compounds such as cephalosporins; 300Å ones fit for
biomacromolecules such as proteins and polypeptides. - Seven pore sizes: 120Å, 200Å, 300Å, 500Å, 700Å, 1000Å and 2000Å.
| Phase | SEC-120 | SEC-200 | SEC-300 | SEC-500 | SEC-700 | SEC-1000 | SEC-2000 |
|---|---|---|---|---|---|---|---|
| Materials | Silica particles bonding hydrophilic polymer | ||||||
| Particle Size (µm) | 3,5 | 3,5 | 3,5 | 5 | 5 | 5 | 5 |
| Pore Size (Å) | 120 | 300 | 300 | 500 | 700 | 1000 | 1000 |
| Protein Molecule Range | 500-150,000 | 500-200,000 | 5,000-1,250,000 | 10,000-3,500,000 | 15,000-5,000,000 | 50,000-7,500,000 | >10,000,000 |
| Soluble Polymer Molecule Mass Range | 500-25,000 | 500-50,000 | 1,000-100,000 | 2,000-500,000 | 2,500-500,000 | 5,000-1,500,000 | 50,000-2,500,000 |
| Maximum Pressure (psi) | ~4,500 | ~4,500 | ~3,500 | ~3,000 | ~3,000 | ~3,000 | ~3,000 |
| pH Range | 2-7.5 (7.5-9.5 for short time) | ||||||
| Range of Salt Concentration | 20mM~2.0M | ||||||
| Highest Temperature (°C) | ~80°C | ||||||
| Mobile Phase | Aqueous or organic phase |
Xtimate® Bio SEC
Xtimate@ Bio SEC column is a size exclusion chromatography column with ultra-pure silica gel as the matrix, and its station- ary phase is a hydrophilic high molecular weight polymer with uniform nano-thickness, bonded evenly on the surface of ultra-pure silica gel microspheres. Welch adopts unique surface modification technology to ensure the complete and uniform bonding of polymer nano-layers on the silica gel surface, greatly covering the silica gel surface, reducing non-spe- cific adsorption of biological samples in the silica gel filler, while possessing good stability and batch reproducibility. The main application fields include biomolecules (such as proteins, nucleic acids, peptides, oligonucleotides), bacteria, viruses, etc.
Features
- The stationary phase consists of hydrophilic high molecular weight polymer with uniform nano-thickness bonded evenly on the surface of ultra-pure silica gel microspheres.
- Available in 5um and 3um silica gel microspheres to meet high-throughput testing requirements.
- Products with different pore size parameters are available to meet various needs for separation and analysis of biomolecules.
- Minimal non-specific adsorption of biomolecular samples.
- Excellent stability and good reproducibility between batches.
| Phase | Bio SEC-100 | Bio SEC-120 | Bio SEC-150 | Bio SEC-200 | Bio SEC-300 | Bio SEC-500 | Bio SEC-1000 |
|---|---|---|---|---|---|---|---|
| Materials | Surface-bonded hydrophilic polymer on silica gel microspheres | ||||||
| Particle Size (μm) | 3μm, 5μm | ||||||
| Pore Size[Å) | 100 | 120 | 150 | 200 | 300 | 500 | 1000 |
| Protein Molecule Range | 100-100,000 | 500-150,000 | 500-200,000 | 1000-500,000 | 5,000-1,250,000 | 15,000-5,000,000 | 50,000-7,500,000 |
| Pressure Tolerance | 100 bar, 5um; 200 bar, 3um | ||||||
| pH Range | 2-8 | ||||||
| column ID | 4.6×300mm; 7.8x300mm | ||||||
| Flow Rate | 0.1~0.4mL/min, 4.6mm inner diameter; 0.3~1.0mL/min, 7.8mm inner diameter | ||||||
| Highest Temperature(C) | 5-60 C |