BLOSSMATE® SEIRES HPLC COLUMN
Blossmate series column is a high-end HPLC column launched by Welch Materials. Compared with Xtimate and Ultisil
series, Blossmate’s column performance and reproducibility have been improved in an all way and it is especially suitable
for the detection of multi-component impurity projects.
Features
- It adopts a new generation of ultra-high-purity fully porous silica gel, which greatly ensures the perfect column efficiency and separation performance.
- Each column is tested individually with special testing prodecure under stricter standards, which guarantee the quality and performance of the column.
- Extremely stable separation performance to ensure better analysis reproducibility and perfect peak shape.
- Excellent batch-to-batch reproducibility, especially for the analysis of multi-impurity component applications.
- Blossmate uses a unique bonding process that enables the column to withstand high water phase, high pH experimental environments.
Blossmate® series silica
Blossmate® series columns use a new high-purity fully porous silica and Welch’s unique bonding process and double end-capping techniques to ensure that the silica surface has a higher inertness, and thus has a more symmetrical peak shape and higher column efficiency. Blossmate columns adopt high-standard strict quality control conditions to ensure that each column has undergone strict quality screening before leaving the factory, which makes the column have better reproducibility and higher peak capacity.
Blossmate® series columns provide better reproducibility, higher efficiency and higher peak capacity
Blossmate series HPLC column uses a new fully porous silica packing materials, which has higher silica purity, more uniform particle size and more uniform pore size distribution. Under the unique packing process and strict quality control conditions, Blossmate silica not only has the high mechanical strength and high column efficiency, but also has the perfect and excellent reproducibility, makes it to be the best choice for highly reproducible project.
| Bonded Phase | Octadecyl Group | Surface Area (m²/g) | 300 (100Å) |
|---|---|---|---|
| Bonded Phase | Octadecyl group | 300 (100Å) | 300 (100Å) |
| pH Range | 2.0-8.0 | Carbon Loading (%) | 14 (100Å) |
| Particle Size | 5um | USP List | L1 |
| Endcapped | Yes | ||
Blossmate® C18
Blossmate® C18 is a general-purpose, highly versatile column which suitable for sample analysis of many complex compo- nents, as well as flexible method development under a range of chromatographic conditions.
| Rt (min) | Area | Area (%) | Peak height | Plates | Resolution (USP) | Asymmetry |
|---|---|---|---|---|---|---|
| 18.584 | 14526 | 1.469 | 806 | 26475 | n.a. | 1.08160 |
| 23.608 | 32974 | 3.334 | 1479 | 28102 | 9.85059 | 1.18897 |
| 24.872 | 7717 | 0.780 | 382 | 32232 | 2.26092 | 0.96729 |
| 30.278 | 156595 | 15.834 | 5161 | 27563 | 8.42344 | 1.44760 |
| 31.970 | 72213 | 7.302 | 2437 | 29831 | 2.30160 | 1.03876 |
| 39.024 | 294693 | 29.798 | 9159 | 33714 | 8.87067 | 1.01296 |
| 41.307 | 351960 | 35.589 | 10469 | 33294 | 2.60093 | 0.95249 |
| 43.794 | 23177 | 2.344 | 610 | 29056 | 2.57241 | 0.98393 |
| 52.527 | 21926 | 2.217 | 487 | 33587 | 8.03340 | 1.08107 |
| 55.686 | 13179 | 1.333 | 294 | 34346 | 2.69108 | 1.13076 |
Column:
Blossmate® C18 4.6x250mm, 5um
Mobile Phase:
Acetonitrile/isopropyl alcohol = 51:49
Flow Rate:
1.0 mL/min
Detector:
Evaporative Light Scattering Detector
Drift Tube Temperature: 25°C, Carrier gas flow rate: 1.5 L/min
Temperature:
30°C
Injection Volume:
5 µL
Ordering Information - Blossmate® C18
P/N
0061-21043
Partical Size
5um
Specification
4.6x250mm
Blossmate® Aqs C18 - High Water-resistance HPLC Column
Blossmate® Aqs C18 is a C18 reversed-phase column compatible with pure water phase and pure salt phase. Under the condition of high proportion of water phase, the column still has excellent stability and high column efficiency, suitable for analysis of hydrophilic and highly polar samples.
| pH range | Carbon load | Pore Size | Specific Surface Area | Maximum Temperature | Maximum Pressure |
| 2.0-8.0 | 10% | 100Å | 300m²/g | 60°C | 40MPa |
| Name | Rt (min) | Area | Peak Height | Plates | Resolution | Tailing Factor |
| 1 | 10.981 | 1433409 | 98041 | 12632 | n.a. | 0.93 |
| 2 Puerarin Peak | 17.684 | 10686918 | 600548 | 23303 | 15.45 | 0.97 |
| 3 | 19.382 | 1928910 | 106398 | 25632 | 3.54 | 1.00 |
| 4 | 22.434 | 1659460 | 85394 | 29652 | 6.01 | 1.02 |
| 24.758 | 2122902 | 101434 | 34075 | 4.33 | 0.91 | |
| 6 Pachaline hydrochloride | 42.708 | 4111863 | 294465 | 207619 | 38.63 | 1.22 |
| 7 | 43.603 | 814122 | 79927 | 399390 | 2.71 | 1.15 |
| 8 | 47.692 | 2510271 | 144775 | 171772 | 10.94 | 0.88 |
Column:
Blossmate® Aqs-C18, 4.6×250mm, 5um
Mobile Phase:
A: Methanol, B: Take 1.5mL of trifluoroacetic acid, add it to 1000mL of water to make a 0.15% trifluoroacetic acid solution, mix well, and ultrasonically degas.
Flow Rate:
1.0 mL/min
Detector:
Characteristic Spectrum 250nm Content Determination Puerarin 250nm Berylkerine Hydrochloride 348nm
Temperature:
30°C
Injection Volume:
10 µL
Gradient Program:
| Time(min) | Mobile Phase A(%) | Mobile Phase B(%) |
| 0 | 23 | 77 |
| 25 | 30 | 70 |
| 26 | 35 | 65 |
| 39 | 42 | 68 |
| 40 | 45 | 55 |
| 55 | 45 | 55 |
Ordering Information - Blossmate® Aqs C18
P/N
00602-21043
Particle Size
5um
Specification
4.6x250mm
Blossmate® ST C18 - Wide pH Range HPLC Column
Blossmate® ST-C18 column adopts a special silica matrix surface treatment technology, while maintaining the high mechanical strength and high column efficiency of the silica matrix, the pH tolerance range of the column is extended to 1.0-11.0, suitable for the analysis of basic samples, and in method development at higher pH conditions.
| pH range | Carbon load | Pore Size | Specific Surface Area | Maximum Temperature | Maximum Pressure |
| 1.0-11.0 | 12% | 100Å | 300m²/g | 60°C | 40MPa |
Column:
BlossmateST-C18, 4.6x250mm, 5um
Mobile Phase:
A: Methanol/Water = 25/75
Flow Rate:
1.0 mL/min
Detector:
250 nm
Temperature:
25°C
Injection Volume:
10 µL
Ordering Information - Blossmate® ST C18
P/N
00603-21043
Particle Size
5um
Specification
4.6x250mm
Blossmate® PSV C18
Blossmate® PSV C18 is a newly developed HPLC column which can be compatible with high proportion of aqueous phase. Taking super high purity spherical silica as matrix, it bonded high-density alkyl functional groups. Its packing materials have high selectivity and strong retention ability for hydrophilic and polar compounds which are often difficult to be retained and separated in normal C18 columns. Blossmate® PSV C18 is fully end-capped, which greatly enhances the packing materi- als’ stability. Even under neutral pH condition, it keeps stable baseline and high sensitivity, making it particularly suitable for high efficiency separation columns with LC-MS. Now, it is widely used in the separation and analysis of oligosaccha- rides, amino acids, small peptides, nucleotides, organic acids and other active components.
Features
- WIth strong separation and retention ability, better peak shape, higher column efficiency.
- Remain stable baseline and high sensitivity even under neutral pH condition.
- Suitable for high efficiency separation columns by LC-MS.
Five food additives
Column:
Blossmate® PSV C18, 4.6×250mm, 5um
Mobile Phase:
20mM ammonium acetate solution:
methanol=93: 7(on-line blending)
methanol=93: 7(on-line blending)
Flow Rate:
1.0mL/min
Detector:
230 nm
Temperature:
23°C
Injection Volume:
5µL
Note: When the Blossmate PSV C18 column is used for the determination of five kinds of food additives, in order to ensure the resolution and the life of the column, the proportion of the water phase in the mobile phase shall not be less than 7%.
P/N
00605-21041
00605-21043
Particle Size
5um
5um
Specification
4.6x250mm
4.6x250mm
Blossmate® PSV C18 Plus - the next generation dedicated column for preservatives
Why is the preservative testing so harmful to HPLC columns?
Complex sample matrix but simple pretreatment.
There are many kinds of food on the market, juice, biscuits, cakes, soy sauce, meat ….. basically all of them contain preserva- tives. But for so many kinds of samples, same pretreatment method is used, which leads to a large amount of small molecu- lar impurities and particulate matter are existed in the test samples, which can easily contaminate the column, resulting in a rapid decline in the column performance.
To resolve this problem, Welch launched Blossmate PSV Plus Column to meet your requirements of preservative testing. Blossmate PSV C18 Plus column adopts the integrated design of the guard column and the analytical column. An integrated guard column is added at the front end of the analytical column to protect the analytical column in all directions. At the same time, the dead volume is small, and the replacement of the cartridge is convenient.
| Name | Rt (min) | Area | Height | R | Plates | Tailing factor |
| Acesulfame | 7.687 | 85170 | 10969 | 22332 | 1.00 | |
| Benzoic acid | 10.096 | 95190 | 9935 | 10.25 | 24980 | 0.96 |
| Sorbic acid | 14.040 | 155694 | 11442 | 12.56 | 24061 | 0.96 |
| Sodium Saccharin | 15.546 | 63598 | 4132 | 3.83 | 22703 | 0.94 |
| Dehydroacetic acid | 18.454 | 150007 | 5520 | 5.27 | 12723 | 1.80 |
Column:
Blossmate® PSV C18, 4.6×250mm, 5um
Mobile Phase:
20mM ammonium acetate solution:
methanol=93: 7(online Mixing)
methanol=93: 7(online Mixing)
Flow Rate:
1.0mL/min
Detector:
230 nm
Temperature:
23°C
Injection Volume:
5µL
Serivce Life Experiment
| Injections | Flow rate (mL/min) | R of dehydroacetic acid & sodium saccharin (R71.50) | Plate (dehydroacetic acid) | Tailing factor (dehydroacetic acid) | Column pressure (MPa) |
| 1st | 1.0 | 5.27 | 12723 | 1.80 | 14.0 |
| 10th | 1.0 | 5.73 | 13344 | 1.78 | 14.0 |
| 50th | 1.0 | 5.92 | 13702 | 1.66 | 14.1 |
| 100th | 1.0 | 5.49 | 14407 | 1.70 | 14.1 |
| 200th | 1.0 | 6.01 | 14604 | 1.67 | 14.3 |
| 300th | 1.0 | 6.41 | 15408 | 1.61 | 14.2 |
| 400th | 1.0 | 5.51 | 14487 | 1.68 | 14.1 |
| 500th | 1.0 | 3.49 | 15917 | 1.83 | 14.3 |
| 600th | 1.0 | 3.83 | 11100 | 1.81 | 14.3 |
| 700th | 1.0 | 3.44 | 8189 | 1.82 | 14.4 |
| 800th | 1.0 | 4.09 | 12662 | 1.38 | 14.2 |
| 900th | 1.0 | 3.98 | 12791 | 1.70 | 14.3 |
| 1000th | 1.0 | 3.96 | 11340 | 1.83 | 14.3 |
Blossmate® PSV C18 Plus - the next generation dedicated column for preservatives
P/N
00605-21441
00605-21443
00808-04143
Specification
4.6x150mm, 5um
4.6x250mm, 5um
Cartridge 4.6x10mm, 5um, 120A
Blossmate® SAX
Blossmate® SAX column can be used under the condition of high flow rate and high pressure. It is compatible with ionic strength change of various mobile phase to achieve fast equilibrium and suitable for the separation and purification of polar small molecules and other biological macromolecules compounds, such as glyphosate, nucleotides, proteins and peptides.
Features
- Based on ultra pure spherical silica gel, bonded quaternary ammonium functional group with high density and high mechanical strength.
- Remain stable baseline and high sensitivity even under neutral pH condition.
- Compatible with organic solvent and mobile phase of buffer salts, remain stable chromatographic properties.
- Comply with the standard of determination of glyphosate, excellent batch to batch stability and long lifetime, ensuring efficient analysis properties.
Specifications
| Bonded phase | Quaternary ammonium functional group | Surface Area(m2/g) | 300(120A) |
| pH Range | 2.0-8.0 | Carbon Loading(%) | 6.5(120Å) |
| Particle Size | 5um | USP List | L14 |
| Endcapped | No |
Column:
Blossmate® SAX, 4.6×250mm, 5um
Mobile Phase:
20mM ammonium acetate solution:
methanol=93: 7(online Mixing)
methanol=93: 7(online Mixing)
Flow Rate:
1.0mL/min
Detector:
195 nm
Temperature:
30°C
Injection Volume:
20µL
Note: after mixing mobile phase well, adjust pH to 2.0.
Ordering Information - Blossmate® SAX
P/N
00605-21041
00605-21043
Particle Size
5um
5um
Specification
4.6x250mm
4.6x250mm
Blossmate® C4
Welch Materials has launched a new Blossmate” C4 column, which fully meets the needs of detecting various biological samples, and provides customers with a HPLC column with higher accuracy, faster peak output, lower cost and a wider range of protein measurement.
Features
- Higher Accuracy: Porous particle silica packing (3.5um) with large pore size (450A) to improve protein resolution.
- Faster peak times: Compared to columns packed with the same size fully porous particles, the analysis time is shorter.
- Lower cost: Stable porous layer packed bed and 2um inlet frit prevent inlet clogging, thus extending column life.
- Wider testing range: measurable protein molecular weight 12kDa-250kDa.
| Name | Bonded Phase | Particle Size | Pore Size | Specific Surface Area | Carbon load | pH Stability | Endcapped |
| Blossmate® C4 | Butylsilane | 3.5um | 450Å | 15m2/g | 0.5% | 1.5-10.0 | Yes |
| Name | Rt (min) | Area | Height | Area% | Plates | Resolution | Tailing Factor |
| RNase A | 5.527 | 1760396 | 387558 | 6.75 | 39328 | n.a. | 1.74 |
| Cytochrome c | 7.662 | 3465735 | 433891 | 13.30 | 27913 | 13.71 | n.a. |
| Lysozyme | 9.170 | 8614697 | 1203650 | 33.05 | 46827 | 8.02 | 2.70 |
| Transferrin | 9.596 | 6962972 | 532132 | 26.71 | 27533 | 1.86 | n.a. |
| A-lactalbumin | 10.790 | 2401376 | 316898 | 9.21 | 91394 | 5.45 | n.a. |
| Catalase | 11.940 | 1458165 | 219515 | 5.59 | 128660 | 7.63 | 3.03 |
| Carbonic Anhydrase | 12.693 | 1400960 | 316344 | 5.38 | 210042 | 5.89 | 1.26 |
Column:
Blossmate® SAX, 4.6×250mm, 5um
Mobile Phase:
A: 0.1% trifluoroacetic acid
B: 0.1% trifluoroacetic acid in acetonitrile
B: 0.1% trifluoroacetic acid in acetonitrile
Flow Rate:
0.3mL/min
Detector:
214nm
Temperature:
60C
Injection Volume:
5uL
Blossmate® Phenyl
Welch Materials has launched a new Blossmate Phenyl column to fully meet the needs of detecting various biological samples, providing customers with higher accuracy, faster peak output, lower cost and a wider range of protein measure- ment columns.
Features
- Higher Accuracy: Porous particle silica packing (3.5um) with large pore size (450A) to improve protein resolution.
- Faster peak times: Compared to columns packed with the same size fully porous particles, the analysis time is shorter.
- Lower cost: Stable porous layer packed bed and 2um inlet frit prevent inlet clogging, thus extending column life.
- Wider testing range: measurable protein molecular weight 12kDa-250kDa.
| Name | Bonded Phase | Particle Size | Pore Size | Specific Surface Area | Carbon load | pH Stability | Endcapped |
|---|---|---|---|---|---|---|---|
| Blossmate® Phenyl | Phenylsilane | 3.5um | 450Å | 15m2/g | 1.0% | 1.5-10.0 | Yes |
| Name | R (min) | Area | Height | Area% | Plates | Resolution | Tailing Factor |
| RNase A | 4.479 | 2740222 | 521395 | 15.26 | 18343 | n.a. | n.a. |
| Cytochrome c | 6.470 | 2369215 | 412742 | 13.19 | 39592 | 14.82 | n.a. |
| Lysozyme | 8.136 | 3443765 | 700496 | 19.18 | 77448 | 13.06 | 2.30 |
| Transferrin | 8.841 | 4900384 | 664186 | 27.29 | 42062 | 3.87 | n.a. |
| A-lactalbumin | 9.339 | 2133699 | 337141 | 11.88 | 75760 | 2.55 | n.a. |
| Carbonic Anhydrase | 11.776 | 2370479 | 425492 | 13.20 | 113433 | 16.90 | 1.88 |
Column:
Blossmate® SAX, 4.6×250mm, 5um
Mobile Phase:
A: 0.1% trifluoroacetic acid
B: 0.1% trifluoroacetic acid in acetonitrile
B: 0.1% trifluoroacetic acid in acetonitrile
Flow Rate:
0.3mL/min
Detector:
214nm
Temperature:
60C
Injection Volume:
5uL