ULTISIL® SERIES HPLC COLUMN
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Ultisil “Ultimate” Series High Performance Liquid Chromatography Columns use ultra-high purity fully porous spherical
silica gel as the matrix. They are produced using the company’s unique stationary phase bonding technology and silica
surface treatment technology, resulting in excellent chromatographic peak shape, separation efficiency, stability, and
reproducibility.
The series offers a complete range of bonded phases, with stable performance, making it the best choice
for a wide range of chromatographers in method development.
Product characteristics
ULTISIL® XB SERIES HPLC COLUMN
Ultisil® XB-C18-Universal HPLC Analytical Column
Ultisil® XB-C18 is the most commonly used column in the market. It can substitute Waters Symmetry C18, Agilent Zorbax XDB C18, Phenomenex Luna C18, Supelcosil LC-18-DB, YMC ODS-AM, Alltima C18, GL, Inertsil ODS-2 etc. XB-C18 has high theoretical plates and peak capacity, so it’s suitable for analysis of complex samples.
Specification
| Structural Formula |  |
| pH Range | 1.5–10.0 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å), 90(300Å) |
| Carbon Loading (%) | 17(120Å), 8(300Å) |
| USP List | L1 |
| Endcapped | Yes |
Ultisil® XB-C8-Less retentive than XB-C18
Ultisil® XB-C8 Phase
The XB-C8 phase is less retentive than XB-C18 phase, useful for strong hydrophobic compounds that are too strongly retained on C18 phase, and for LC/MS applications, where long retention is not desired. When separating neutral or other highly retained compounds, XB-C8 can save analytical time. However, when separating polar compounds, XB-C8 column provides different selectivity than does XB-C18 column.
Specification
| Structural Formula | |
| pH Range | 1.5–10.0 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å), 90(300Å) |
| Carbon Loading (%) | 12(120Å), 4(300Å) |
| USP List | L7 |
| Endcapped | Yes |
Ultisil® XB-Phenyl-Different Selectivity to Alkyl Phase
Ultisil® XB-Phenyl Phase
Ultisil® XB-Phenyl phase is less retentive than conventional C18 or C8 phases, but more retentive than standard cyano phase. Due to their ability to participate in Tt-Tt interactions, XB-Phenyl columns may actually be more retentive than C18 or C8 columns towards certain polar aromatic compounds, depending on running conditions. The selectivity for highly polar aromatics, which are poorly retained on alkyl-bonded phases, together with reduced retentivity towards non-polar compounds, make XB-Phenyl an excellent choice for the analysis of complex mixtures of polar and non-polar analytes. Additionally, this bonding phase boasts high surface coverage and exhaustive double end-capping, leading to better performance.
Specification
| Structural Formula |  |
| pH Range | 1.5–10.0 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å), 90(300Å) |
| Carbon Loading (%) | 12(120Å), 4(300Å) |
| USP List | L7 |
| Endcapped | Yes |
Ultisil® XB-C4-suitable for separation of bio-samples
Features
- Optimize the packing process, employ an improved production method for a more effective capture, and longer durability.
- Column packing of 300Å big pore size particles is appropriate for separation of peptide and protein samples with sharp peak shape.
- Minibore column can be used for LC/MS(/MS).
Specification
| Structural Formula |  |
| pH Range | 1.5–10.0 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å), 90(300Å) |
| Carbon Loading (%) | 8(120Å), 3(300Å) |
| USP List | L26 |
| Endcapped | Yes |
Ultisil® XB-C1
Ultisile XB-c1 column
Ultisile XB-C1 column is bonded with trimethylchlorosilane, possessing medium polarity. It can be used with common reverse-phase solvents to analyze hydrophobic compounds or with high-water content solvents to analyze highly polar compounds. Due to hydrophobic interactions, the retention is lower compared to other high-performance liquid chroma- tography columns, enabling rapid elution of hydrophobic compounds. Sometimes, it can also provide better separation of hydrophilic compounds than other reverse-phase columns. It is suitable for separating samples that are highly polar and difficult to separate with general reverse-phase or normal-phase columns.
Specification
| Structural Formula |  |
| pH Range | 1.5–10.0 |
| Particle Size | 5µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | 4(120Å) |
| USP List | L13 |
| Endcapped | Yes |
Features
- Lowest hydrophobicity among reversed phases.
- Intermediate polarity between normal phase silica and other alkyl bonded reversed phase.
- Alternative selectivity to C18 phase..
Ultisil® XB-CN-unique selectivity for polar compounds
Ultisil* xB-CN column
Ultisil* XB-CN column can be used in either reversed or normal phase. Reversed phase CN column has special selectivity
for polar compounds, and due to its low hydrophobicity, elution of hydrophobic molecules is fast. Furthermore, XB-CN
column shows perfect peak shape for strong basic analytes (induding quaternary ammonium salts), Polarity of XB-CN
column is the strongest among all reversed columns. It is a good choice for compounds that are strongly retained on
standard reversed columns.
Normal phase CN column can replace SiO2 column. Equilibrium of normal phase column is fast, and the silica surface activi-
ty is better than that of silica column. To prolong column life time, alternation between reversed phase and normal phase
uses should be avoided. While XB-CN column can be used in either reversed or normal phase, elution sequence is different
in different separation mode.
Specification
| Structural Formula | |
| pH Range | 1.5–10.0 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | 7(120Å) |
| USP List | L10 |
| Endcapped | Yes |
Features
- Can be used in either reversed or normal phases.
- Stable bonding chemistry and excellent surface coverage.
- Low hydrophobicity, unique selectivity.
Ultisil® SiO2
Ultisile sio2 column
Ultisil SiO2 column uses ultra-high purity type B silica particles with no metal contents. SiO2 column can separate strong hydrophilic compounds in high concentration organic solvent in reversed phase. Good result can be obtained for the analysis of polar compounds which are prone to peak tailing in reversed phase.
Specification
| Structural Formula |  |
| pH Range | 2.0–8.0 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å), 90(300Å) |
| Carbon Loading (%) | N/A |
| USP List | L3 |
| Endcapped | Yes |
Ultisil® HILIC Diol
Specification
| Structural Formula |  |
| pH Range | 2.0–8.0 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | 2.5(120Å) |
| USP List | L20 |
| Endcapped | NO |
Ultisil® HILIC Amphion II
Ultisil® HILIC Amphion II
Ultisil® HILIC Amphion II is a newly developed HILIC column, using amphion-bonded silica as packing material. It applies to the separation of most polar compounds, using acetonitrile or Water other than ion-pairing reagents as mobile phase. The Amphion, containing both Positive Charge Centre and Negative Charge Centre, brings high retention for acid and alkaline compounds through ion-exchange mechanism. Compared with common HILIC packing materials like silica and amino groups, the Amphion-bonded packing material provides better reproducibility and stability.
Specification
| Structural Formula |  |
| pH Range | 2.0–8.0 |
| Particle Size | 5µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | N/A |
| USP List | L114 |
| Endcapped | N/A |
Features
- Amphion-bonded silica stationary phase.
- Enhanced hydrophilic interaction brings higher retention for polar and hydrophilic compounds.
- Different selectivity from common HILIC packing materials.
- Simple mobile phase used for the separation of polar compounds.
ULTISIL® MIXED MODE PHASE HPLC COLUMN
| Structural Formula | |
| pH Range | 2.0–8.0 |
| Particle Size | 5µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | N/A |
| USP List | L114 |
| Endcapped | N/A |
ULTISIL® MM C18/SCX
Mixed Mode Phase, as a novel packing material, exhibits dual mechanisms of hydrophobic and ion exchange actions, providing distinct selectivity compared to traditional single-bonded phases. It is considered one of the trends in the future development of the liquid chromatography column industry.
Specification
| pH Range | 2.0–8.0 |
| Particle Size | 5µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | N/A |
| USP List | / |
| Endcapped | N/A |
Features
- Utilizes high-purity spherical porous silica gel as the matrix.
- C18 and SCX mixed bonding ratio is 4:1.
- Applicable for separating and analyzing hydrophobic and ionized compounds.
- Ideal for the analysis of unknown compounds, particularly in metabolite research.
ULTISIL® CHIRAL HPLC COLUMN
Ultisil® Cellu-D/Cellu-DR
Ultisil® Chiral Columns are based on spherical silica particles coated with chiral polymers (amylose derivatives or cellulose derivatives). Welch offers 5 um and 10 um particles, and four types of chiral columns: Cellu-D, Cellu-J, Amy-D and Amy-s. 80% of all racemic compounds can be separated by these four chiral columns.
Specification
| Structural Formula |  |
| pH Range | 2.0–8.0 |
| Particle Size | 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | N/A |
| USP List | L40(Cellu-D), L93(Cellu-DR) |
| Endcapped | N/A |
ULTISIL® SPECIALIZED C18 HPLC COLUMN
Ultisil® AQ-C18-The most widely used column in food industry
Ultisil® AQ-C18 columns are designed to have extended retention and selectivity for hydrophilic and polar compounds, which are poorly or not at all retained on other phases. A proprietary bonding chemistry, Ultisil AQ-C18 avoids so-called “phase collapse”, even when 100% water is used, a phenomenon that conventional C18 columns typically exhibit at high water content in the mobile phase. Ultisil® AQ-C18 phase is fully end-capped to ensure the best peak shapes of polar and basic compounds and longer lifetime. Typical applications are separations of water soluble compounds that cannot be retained on traditional C18 phase. Examples include biomolecules, metabolites, and pharmaceutical degradants such as organic acids, water-soluble vitamins, oligosaccharides, amino acids, and small peptides and nucleotides.
Specification
| Structural Formula |  |
| pH Range | 1.5–10.0 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | 12(120Å) |
| USP List | L1/L96 |
| Endcapped | Yes |
Ultisil® Amino Acid
Ultisil® Amino Acid
Ultisil® Amino Acid HPLC columns are made from spherical, totally porous, and ultra-high purity (>99.999%) type B silica particles. Our proprietary surface modification before bonding generates a very smooth and uniform surface with less acidic surface silanol. Ultisil” Amino Acid columns provide the best performance in peak shape, efficiency and resolution for the analysis of 18 amino acids. Complete sample preparation can be achieved in as short as 30 min.
Specification
| Structural Formula |  |
| pH Range | 1.5–10.0 |
| Particle Size | 3µm, 5µm, 10µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | 12(120Å) |
| USP List | L1/L96 |
| Endcapped | Yes |
Ultisil® Amino Acid Method Package
- Ultisil® Amino Acid Column (5um, 4.6x250mm), 1 pk.
- Amino Acid Standards, 2 bottles. 1 mL/bottle.
- Derivatization reagent A.
- Derivatization reagent B.
- Ultisil® AA method brochure.
| Name | P/N | Description |
|---|---|---|
| Ultisil® Amino Acid Method Package (P/N 00840-01000) | H00211-31043 | Ultisil® Amino Acid Column (4.6×250mm, 5µm), 1 pk |
| 00814-01027 (A) | Derivatization reagent A, 1 bottle, 10mL/bottle | |
| 00814-01027 (B) | Derivatization reagent B, 1 bottle, 10mL/bottle | |
| 00814-01030 | Derivatization reagent diluent, 6 bottles, 20mL/bottle | |
| 00815-01001 | Amino Acid Standards, 2 bottles, 1mL/bottle |
Ultisil® Amino Acid Plus
Ultisil® Amino Acid Plus
Ultisil® Amino Acid Plus column is a dedicated column which through further optimizing the analysis method on the basis of the original column for amino acid analysis. It uses an evaporative light scattering detector to detect more kinds of amino acids with higher stability without derivation of amino acid.
Specification
| pH Range | 1.0-7.0 |
| Particle Size | 5µm |
| Surface Area (m²/g) | 320(120Å) |
| Carbon Loading (%) | 10(120Å) |
| USP List | L1 |
| Endcapped | Yes |
Features
- Separate 23 amino acids by reverse-phase chromatographic analysis without the need of derivation, which makes amino acid analysis more convenient and flexible.
- Amino acids which separated and derived from proteolytic products, cell culture medium, food and feed have better resolution.
- The special column for amino acid analysis has superb reproducibility and stability, ensuring the stability and reliability of quantitative and qualitative analysis results.
- Excellent selectivity and separation, allowing you to get more accurate analysis results.
- Multiple interference factors such as reagents, by-products and solvents can be removed by fast extraction.
- Adhere to strict quality control standards, each column had been tested with 23 amino acids before sold, ensuring the reliability of the results.
separation of 23 Amino Acids
| Name | Description |
|---|---|
| H00279-31044 | Ultisil® Amino Acid Column (4.6×250mm, 5µm), 1 pk |